Journal: Redox Biology

Article Title: Endothelial Lon protease 1 facilitates the redox balance to prevent glomerulosclerosis by acting on superoxide dismutase 2 ubiquitination

doi: 10.1016/j.redox.2025.103929

Figure Lengend Snippet: SOD2 supplementation alleviates glomerulosclerosis and inhibits the vascular endothelial cells inflammation response. A) Masson's trichrome staining in different groups ( n = 8–9, Scale bar, 50 μm). B, C) Fibrotic area of glomerulus (B) and tubules (C) statistics of Masson's trichrome staining in different groups ( n = 8–9). D) PAS staining in different groups ( n = 8–9, Scale bar, 50 μm). E) Systolic blood pressure in different groups detected by tail-cuff ( n = 8–10). F) ELISA of urinary microalbumin in different groups ( n = 8–10). G) Analysis of Cr in different groups ( n = 8–9). H) Relative fluorescence intensity statistics of IF staining of Fibronectin ( n = 5). I) IF staining of Fibronectin in different groups ( n = 5, Scale bar, 20 μm). J, K) Western blot of Fibronectin in different groups. GAPDH was used as a control ( n = 4). Dot plots represent quantitative densitometric data from Western blot. L) IHC staining of F4/80 in different groups ( n = 5, Scale bar, 50 μm). M) IHC semi-quantitative IOD analysis of F4/80 ( n = 5). N, O) Western blot of CD31, VCAM-1, and ICAM-1 in immortalized HAECs transfected with shRNA of LONP1 and treated with MnTBAP. β-Actin was used as a control ( n = 3). Dot plots represent quantitative densitometric data from Western blot. P, Q) Western blot of CD31, VCAM-1, and ICAM-1 in MAECs transfected with shRNA of LONP1 and treated with MnTBAP. GAPDH was used as a control of CD31, β-Actin was used as a control of VCAM-1 and ICAM-1 ( n = 3). Dot plots represent quantitative densitometric data from Western blot. R, S) The IL-6 (R) and TNF-α (S) levels of cell medium in MAECs transfected with shRNA of LONP1 and treated with MnTBAP were detected by ELISA ( n = 3). T, U) Western blot of CD31, VCAM-1, and ICAM-1 in immortalized HAECs co-transfected with shRNA of LONP1 and SOD2 overexpression plasmid. β-Actin was used as a control ( n = 3). Dot plots represent quantitative densitometric data from Western blot. V, W) Western blot of CD31, VCAM-1, and ICAM-1 in MAECs co-transfected with shRNA of LONP1 and SOD2 overexpression plasmid. GAPDH was used as a control of CD31, β-Actin was used as a control of VCAM-1 and ICAM-1 ( n = 3). Dot plots represent quantitative densitometric data from Western blot. X, Y) The IL-6 (X) and TNF-α (Y) levels of cell medium in MAECs co-transfected with shRNA of LONP1 and SOD2 overexpression plasmid were detected by ELISA ( n = 3). IOD, Integral Optical Density.

Article Snippet: Cells were treated with 50 μM MnTBAP (MCE, HY-126397) for 12 h and then transfected with shRNA of LONP1 or treated with Ang II.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Fluorescence, Western Blot, Control, Immunohistochemistry, Transfection, shRNA, Over Expression, Plasmid Preparation

Journal: Toxicology Reports

Article Title: MnTBAP, a peroxynitrite scavenger, attenuates cisplatin-induced apoptosis and cytotoxicity in organ of Corti cells

doi: 10.1016/j.toxrep.2025.101967

Figure Lengend Snippet: Effect of MnTBAP cotreatment on the viability of cisplatin-treated UB/OC-1 cells. A) Dose-dependent cytotoxicity in UB/OC-1 cells after cisplatin treatment. B) Cell viability assay of UB/OC-1 cells after treatment with cisplatin, MnTBAP+Cisplatin, and MnTBAP alone indicates that the cisplatin-mediated cell death was attenuated with MnTBAP cotreatment Results are expressed as mean ± standard deviation, n = 7. ## p < 0.01 vs cisplatin.

Article Snippet: UB/OC-1 cell lines were plated and treated after 24 hours with 100 μM MnTBAP (ChemCruz, sc-221954A, Santa Cruz, CA), one hour prior to cisplatin treatment.

Techniques: Viability Assay, Standard Deviation

Journal: Toxicology Reports

Article Title: MnTBAP, a peroxynitrite scavenger, attenuates cisplatin-induced apoptosis and cytotoxicity in organ of Corti cells

doi: 10.1016/j.toxrep.2025.101967

Figure Lengend Snippet: Effect of MnTBAP cotreatment on cisplatin-induced nitrative stress. A) Immunocytochemistry analysis with anti-nitrotyrosine was used to assess the nitrative stress in UB/OC-1 cells. Magenta staining indicates immunoreactivity to anti-nitrotyrosine while blue indicates nuclear staining with DAPI. B) Quantification of the immunostaining suggested that the level of 3-NT expression increased significantly with cisplatin treatment which was attenuated by MnTBAP cotreatment. The results are expressed as mean ± standard error, n = 6, ****p < 0.0001 vs control, #### p < 0.0001 vs cisplatin, scale bar = 20 µm.

Article Snippet: UB/OC-1 cell lines were plated and treated after 24 hours with 100 μM MnTBAP (ChemCruz, sc-221954A, Santa Cruz, CA), one hour prior to cisplatin treatment.

Techniques: Immunocytochemistry, Staining, Immunostaining, Expressing, Control

Journal: Toxicology Reports

Article Title: MnTBAP, a peroxynitrite scavenger, attenuates cisplatin-induced apoptosis and cytotoxicity in organ of Corti cells

doi: 10.1016/j.toxrep.2025.101967

Figure Lengend Snippet: Effect of MnTBAP cotreatment on cisplatin-induced changes in the expression levels of apoptotic genes. A) Heat map depicts differentially expressed apoptotic genes in UB/OC-1 cells. The expression levels of genes in each treatment group were normalized with control. B) Cisplatin treatment induced the upregulation of many pro-apoptotic genes, while MnTBAP cotreatment attenuated their expression. MnTBAP cotreatment also upregulated the expression of anti-apoptotic genes. Results expressed as mean ± standard deviation, # p < 0.05, ## p < 0.01, n = 4.

Article Snippet: UB/OC-1 cell lines were plated and treated after 24 hours with 100 μM MnTBAP (ChemCruz, sc-221954A, Santa Cruz, CA), one hour prior to cisplatin treatment.

Techniques: Expressing, Control, Standard Deviation

Journal: Toxicology Reports

Article Title: MnTBAP, a peroxynitrite scavenger, attenuates cisplatin-induced apoptosis and cytotoxicity in organ of Corti cells

doi: 10.1016/j.toxrep.2025.101967

Figure Lengend Snippet: Effect of MnTBAP cotreatment on cisplatin-induced changes in the expression levels of activated Caspase-3. A) Immunocytochemistry analysis of UB/OC-1 cell lines with anti-caspase-3 was used to assess apoptosis. Orange staining indicates immunoreactivity to anti-Caspase-3 while blue indicates nuclear staining with DAPI. B) Quantification of the staining intensity suggested that the level of activated Caspase-3 expression increased significantly with cisplatin treatment which was attenuated by MnTBAP cotreatment. The results are expressed as mean ± standard error, n = 6, ****p < 0.0001 vs control, #### p < 0.0001 vs cisplatin, scale bar = 20 µm.

Article Snippet: UB/OC-1 cell lines were plated and treated after 24 hours with 100 μM MnTBAP (ChemCruz, sc-221954A, Santa Cruz, CA), one hour prior to cisplatin treatment.

Techniques: Expressing, Immunocytochemistry, Staining, Control

Journal: Toxicology Reports

Article Title: MnTBAP, a peroxynitrite scavenger, attenuates cisplatin-induced apoptosis and cytotoxicity in organ of Corti cells

doi: 10.1016/j.toxrep.2025.101967

Figure Lengend Snippet: Effect of MnTBAP cotreatment on cisplatin-induced decrease in LMO4 levels. A) Immunoblot analysis with anti-LMO4 was used to assess LMO4 protein levels in UB/OC-1 cells. Actin was used for normalization. B) Quantification of the bands indicated that cisplatin treatment reduced LMO4 protein levels, while cotreatment with MnTBAP attenuated the cisplatin-mediated decrease in LMO4 levels. The results are expressed as mean ± standard error, n = 4, **p < 0.01 vs control, #### p < 0.01 vs cisplatin.

Article Snippet: UB/OC-1 cell lines were plated and treated after 24 hours with 100 μM MnTBAP (ChemCruz, sc-221954A, Santa Cruz, CA), one hour prior to cisplatin treatment.

Techniques: Western Blot, Control